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Heparin / LMWH Testing

EuTech Scientific Services offers expert Heparin and Low Molecular Weight Heparin analysis using a variety of analytical methods, combination of these methods, and customized methods.

Please take a look at our critical discussion of Molar Ratio of Sulfate to Carboxylate presented at the USP 6th Workshop on the Characterization of Heparin Products.

Testing Available for Heparin and LMWH include:

  • Chromatographic Identity
  • Antifactor Xa and Antifactor IIa Ratio and Potency
  • aPTT Test
  • HepTest
  • Molecular weight determination, distribution and weight average molecular weight
  • Chain Mapping
  • Fragment Mapping
  • 1,6 Anhydro Derivative Testing
  • Sequence of Natural Disaccharide
  • Molar Ratio of Sulfate to Carboxylate
  • Free Sulfate Content
  • Ultra Violet Absorption
  • Absence of Over Sulfated Chondroitin Sulfate
  • Heavy Metals
  • Limit of Galacosamine in total hexosamine
  • Nitrogen Determination
  • Nucleotidic Impurities
  • Protein Impurities
  • Lipid Impurities
  • 1H NMR
  • 13C NMR

Please contact us for more information and consultation. All work is performed in strict confidentiality.

Capillary Electrophoresis (CE)

Invited guest lecture on “Validation of a CE Method for Determination of OSCS Contamination in Low Molecular Weight Heparin, Enoxaparin.” at CE Pharm 2009 in Boston on October, 2009  CE of Enoxaparin (Low Molecular Weight Heparin)

+ OSCS Impurity

Strong Ion Exchange (SAX) Chromatography Tests A & B

Test A: SAX onlyTest B: SAX + Nitrous Acid Treatment* + SAX*Nitrous acid de-polymerizes heparin but not OSCS. Zheng et al., Journal of Medicinal Chemistry 2008, 51, 5498.  

 SAX HPLC of Heparin, OSCS, Dermatan Sulfate

Proton NMR (1H NMR)

Heparin: 2.04 ppmOSCS: 2.16 ppm